Fecal Extraction Device


For extraction we recommend the use of Calpro EasyExtract™ or one of the other methods described below. Perform extraction according to package insert for the chosen extraction device/method. Other methods and devices, validated by the customer, can be use.

1. Extraction using the Fecal Extraction Device

Instructions for use: please read package insert for product No. CAL0500

The Fecal Extraction Device is assembled during the extraction procedure as described and illustrated by the pictures below:

  1. Use the small spatula provided to fill the beaker. Alternatively, an inoculation loop can be used. The beaker will take about 100 mg sample. Grains and seeds should be avoided. Also avoid trapping air bubbles.
  2. Wipe off excess sample and push the cap tightly into the cone of the extraction tube lower end.
  3. Fill the extraction tube with 4.9 ml extraction buffer and attach the top cap.
  4. Vortex the assembled device vigorously for about three minutes to disrupt large particles. If necessary, further vortexing may be performed so that only solid particles remain.
  5. Allow particles to settle by leaving the tube on the bench for a couple of minutes. No centrifugation is necessary, but a short centrifugation can be performed if a particle-free solution is required.
  6. Transfer about 0.5 mL to a new tube for assay or storage. Extracts can be stored at 2 – 8°C for at least five days or frozen below -20°C for up to six months 1).
  7. The extract is now ready for dilution according to the package insert of the assay to be used.
  8. The extract represents a 1:50 dilution (weight:volume) of the stool sample.

CLICK HERE FOR PI

2. Extraction using the Calpro EasyExtract™

 Instructions for use: please read package insert for product No. CAL0510

The procedure for extraction of Calprotectin using the Calpro EasyExtract™ device is illustrated by the pictures below:

  1. Take out the required amount of EasyExtract™ devices from the box and equilibrate to room temperature before use.
  2. Hold the blue adaptor in place and release the stick by rotating the red cap counter-clockwise.
  3. Take out the white stick, attached to the red cap.
  4. Dip the stick into the stool sample in three different places, making sure that the grooves of the stick are filled with stool. Grains and seeds should be avoided. Also avoid trapping air bubbles.
  5. Return the stick through the hole in the blue adaptor on the tube. By inserting the stick into the tube, excess stool is wiped off in the funnel insert. Push together until it stops (see figure 4 and 5).
  6. Turn the red cap clockwise until it clicks into the locked position. Make sure that both the blue adaptor and the red cap are in the locked position.
  7. Vortex the assembled device vigorously for about three minutes to disrupt large particles. The stool material should be suspended completely in extraction buffer. If necessary, further vortexing may be performed so that only solid particles remain and the grooves are free of faeces.
  8. Allow particles to settle by leaving the tube on the bench for a couple of minutes. A short centrifugation can be performed if a particle-free solution is required.
  9. Extracts can be stored in the extraction tube at 2 – 8°C for up to five days or frozen below -20°C for up to six months1).
  10. The extract is now ready for dilution according to the package insert of the assay to be used. The extract represents a 1:50 dilution (weight:volume) of the stool sample.
  11. For analysis, open the tube by twisting the blue adaptor counter-clockwise and removing both the adaptor, the red cap and the stick.

3. Extraction using the weighing method (without extraction Device)

  1. Weigh (tare) an empty screw cap tube with an inoculation loop.
  2. Take out approx. 100 mg (between 40 and 120 mg) faeces by means of the inoculation loop and place it into the screw cap tube. Avoid taking out solid, undigested material like fibres and seeds.
  3. Weigh tube and loop with faeces which will give the net faeces weight.
  4. Break or cut off the top half of the loop handle and leave the bottom part inside the tube.
  5. Add extraction buffer to a weight: volume ratio 1:50, for instance 4.9 mL buffer to 100 mg faeces. Close the tube.
  6. Mix vigorously for 30 seconds by means of a vortex mixer.
  7. Continue the mixing on a shaker (at approx. 1000 rpm) for 30±5 minutes with the loop inside the tube as an agitator.
  8. Allow a couple of minutes on the bench for particles to settle and pipette carefully from the top of the tube. No centrifugation is necessary, but a short centrifugation can be performed if a particle-free solution is required.
  9. The extract, which represents a 1:50 dilution (weight:volume) of the stool sample, is now ready for dilution and testing.
  10. For storage, transfer about 0.5 mL to a new tube. Extracts can be stored at 2 – 8°C for at least five days or frozen below -20°C for up to 2 years 48).

CLICK HERE FOR FAEACES SAMPLE COLLECTION AND PREPARATION:

http://phical.com/stool-samples

 

CALPRO TEAM

  • Anne Thjømøe

    Executive Chairman 

    Mail: athjomoe@calpro.no

    Direct line: +47 97 73 37 51

  • Inge Dale

    M.D., Ph.D. Chief Medical Officer

    E-mail: idale@calpro.no

    Direct line: +47 91 31 45 03

  • Anne Hancke Framstad

    Laboratory Quality Specialist

    E-mail: aframstad@calpro.no

    Direct line: +47 92 01 14 91

  • Einar Mørk

    Dir. Operations

    E-mail: einar@calpro.no

    Direct line: +47 47 28 55 61

  • Mona Schartum

    Production Manager

    E-Mail: mona.schartum@calpro.no

    Direct line: +47 93 22 73 63

  • Ingvild haukø bilde

    Ingvild Haukø

    Sales- and Marketing Manager

    e-mail: ingvild@calpro.no

    Direct line: +47 92 01 52 18

  • Ali Qamar

    Product Manager

    E-mail: ali@calpro.no

    Direct line: +47 41 17 81 19

General Information

Fecal calprotectin in differential diagnosis of irritable bowel syndrome

Li XG, Lv YM, Gu F, Yang XL., Department of Gastroenterology, Peking University Third May 9, 2015

PhiCal Test FDA approved

The PhiCal Test has now been approved by the FDA for clinical use in the USA October 1, 2014

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